混入蛹虫草的谷物混合物对小鼠抗疲劳的评价
Context: Cordyceps sinensis (Berk.) Sacc. (Clavicipitaceae) is a famous medicinal fungus (mushroom) in Chinese herbal medicine. Polysaccharides from Cordyceps sinensis (CSP) have been identified as active ingredients responsible for its biological activities. Although many pharmacological actions of CSP have received a great deal of attention, research in this area continues.
Objective: The current study was designed to investigate the effects of CSP on exhaustive exercise-induced oxidative stress.
Materials and methods: The mice were divided into four groups: control (C), low-dose CSP treated (LC), intermediate-dose CSP treated (IC) and high-dose CSP treated (HC). The treated groups received CSP (100, 200 and 400 mg/kg, ig), while the control group received drinking water for 28 days, followed by being forced to undergo exhaustive swimming exercise, and some biochemical parameters including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), malondialdehyde (MDA) and 8-hydroxy-20-deoxyguanosine (8-OHdG) were measured using detection kits according to the manufacturers’ instructions.
Results: Compared with the C group, exhaustive swimming time was significantly prolonged in the LC, IC and HC groups (p50.05); SOD activities in serum, liver and muscle were significantly higher in the IC and HC groups (p50.05); GPx activities in serum, liver and muscle were significantly higher in the LC, IC and HC groups (p50.05); CAT activities in serum, liver and muscle were significantly higher in the HC groups (p50.05); MDA and 8-OHdG levels in serum, liver and muscle were significantly lower in the LC, IC and HC groups (p50.05).
Discussion and conclusion: The results obtained herein indicate that CSP could ameliorate exhaustive exercise-induced oxidative stress.
Keywords
8-Hydroxy-20-deoxyguanosine, catalase, exhaustive swimming time, glutathione peroxidase, liver, malondialdehyde, mice, muscle, serum, superoxide dismutase
Feng Yan1, Beibei Wang1, and Yan Zhang2
1Department of Physical Education, University of International Business and Economics, Beijing, P.R. China and
2Harbin Institute of Physical Education, Harbin, P.R. China
History
Received 18 March 2013
Revised 15 June 2013
Accepted 25 June 2013
Published online 18 September 2013
ISSN 1388-0209 print/ISSN 1744-5116 online Editor-in-Chief: John M. PezzutoMaterials and methods: The mice were divided into four groups: control (C), low-dose CSP treated (LC), intermediate-dose CSP treated (IC) and high-dose CSP treated (HC). The treated groups received CSP (100, 200 and 400 mg/kg, ig), while the control group received drinking water for 28 days, followed by being forced to undergo exhaustive swimming exercise, and some biochemical parameters including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), malondialdehyde (MDA) and 8-hydroxy-20-deoxyguanosine (8-OHdG) were measured using detection kits according to the manufacturers’ instructions.
Results: Compared with the C group, exhaustive swimming time was significantly prolonged in the LC, IC and HC groups (p50.05); SOD activities in serum, liver and muscle were significantly higher in the IC and HC groups (p50.05); GPx activities in serum, liver and muscle were significantly higher in the LC, IC and HC groups (p50.05); CAT activities in serum, liver and muscle were significantly higher in the HC groups (p50.05); MDA and 8-OHdG levels in serum, liver and muscle were significantly lower in the LC, IC and HC groups (p50.05).
Discussion and conclusion: The results obtained herein indicate that CSP could ameliorate exhaustive exercise-induced oxidative stress.
Keywords
8-Hydroxy-20-deoxyguanosine, catalase, exhaustive swimming time, glutathione peroxidase, liver, malondialdehyde, mice, muscle, serum, superoxide dismutase
Feng Yan1, Beibei Wang1, and Yan Zhang2
1Department of Physical Education, University of International Business and Economics, Beijing, P.R. China and
2Harbin Institute of Physical Education, Harbin, P.R. China
History
Received 18 March 2013
Revised 15 June 2013
Accepted 25 June 2013
Published online 18 September 2013
Pharm Biol, 2014; 52(2): 157–161
2014 Informa Healthcare USA, Inc. DOI: 10.3109/13880209.2013.820197
原文链接：/pdf/Polysaccharides from Cordyceps sinensis mycelium ameliorate exhaustive swimming exercise induced oxidative stress.pdf
